|
KMID : 0364819910290040263
|
|
Korean Journal of Microbiology
1991 Volume.29 No. 4 p.263 ~ p.266
|
|
|
Growth and Physiology of Thiobacillus novellus under Autotrophic and Heterotrophic Conditions
|
|
Park In-Kook
|
|
|
Abstract
|
|
|
1
The growth of T. novellas in autotrophic and heterotrophic media was studied to determine the time required for cells to enter stationary phase and relative percentage of ribosomal proteins. When T. novellas was grown autotrophically, growth proceeded at a slow rate characteristic of autotrophs and did not enter log phase until the end of the first day. Logarithmic growth proceeded for 3-4 days at which time the cells entered the stationary phase. In particular, logarithmic growth was accompanied by decreasing pH of culture media and in the stationary phase the pH levelled off at 6.0, a decrease of 1.6 pH value compared to original pH of media. The pH decrease was greatest during log phase when cells oxidized thiosulfate to H2SO4. The doubling time was about 26h. In heterotrophic media growth proceeded at a much faster rate and cells entered stationary phase 20-22h after inoculation. The doubling time was 3 h. The protein content of the ribosomes in T. novellas grown heterotrophically was 4.2% greater than those from the organism grown autotrophically.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|